Reproduction Abstracts

Evaporative-drying is one alternative to cryopreservation. It has the advantages that during sample preparation it does not require liquid nitrogen and a freeze-dryer machine; however, the results have not been as successful as freeze-drying. The objectives of this study were to determine whether artificial activation (electrical and chemical activation) could improve the development of porcine embryos in vitro after intracytoplasmic injected with evaporative-dried sp...

This study was designed to investigate whether acteoside (antioxidant) improves canine SCNT efficiency. Cell cycle of canine fetal fibroblasts was synchronized by culturing to contact inhibition, serum starvation and treating with acteoside. Cell cycle synchronization, apoptosis and reactive oxygen species (ROS) detection were analyzed using flow cytometry. The fibroblasts, prepared by confluent-cell culture or treating with 30 μM acteoside for 48 h, were reconstructed in...